An inverse correlation between RSF1, RUNX2 and miR-154 expression in BC tissues. (A) The expression of RSF1 mRNA was significantly increased in BC tissues when compared to corresponding normal tissue; *P<0.05 vs. normal tissue. (B) The expression of RUNX2 mRNA was also increased in BC tissue compared to normal tissue; *P<0.05 vs. normal tissue. (C) Western blot analyses showing the expression levels of RSF1 and RUNX2 protein in four normal (C1-4) and eight BC samples (B1-8). (D) The relationship between miR-154 and RSF1 mRNA in BC tissue was assessed by Pearson correlation; r=0.5644, P<0.001. (E) The relationship between miR-154 and RUNX2 mRNA in BC tissue; r=0.6208, P<0.001. (F) The relationship between RSF1 and RUNX2 mRNA in BC tissue; r=0.016, P=0.893. RSF1, remodeling and spacing factor 1; RUNX2, runt-related transcription factor 2; BC, bladder cancer.