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. 2017 Sep 19;38(5):2761–2773. doi: 10.3892/or.2017.5969

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Expression levels of VEGF-C, VEGF-D, and VEGFR3 in the lung adenocarcinoma A549 cell line. (A) RT-PCR evaluations of VEGF-C mRNA, VEGF-D mRNA and VEGFR3 mRNA in co-cultured M2 macrophages (CO-M2) and co-cultured A549 cells (CO-A549) are presented. (B) IHC demonstrating that VEGFR3 expression in human lung adenocarcinoma is primarily found in tumor cells than in stromal and adjacent cells. VEGFR3 expression was also found in blood vessels. Scale bars, 20 µm. (C) Semi-quantitative RT-PCR and qRT-PCR evaluation of VEGF-C mRNA, VEGF-D mRNA and VEGFR3 mRNA are presented. The co-cultured A549 cells (CO-A549) had a higher expression level of VEGF-C mRNA and VEGF-D mRNA than that of the A549 cells alone. (D) Western blot analysis confirmed a high protein expression of VEGF-C and VEGFR3 in CO-A549 cells. *P<0.05, **P<0.01, ***P<0.001. VEGFR, vascular endothelial growth factor receptor; IHC, immunohistochemistry.

Figure 3. — Expression levels of VEGF-C, VEGF-D, and VEGFR3 in the lung adenocarcinoma A549 cell line. (A) RT-PCR evaluations of VEGF-C mRNA, VEGF-D mRNA and VEGFR3 mRNA in co-cultured M2 macrophages (CO-M2) and co-cultured A549 cells (CO-A549) are presented. (B) IHC demonstrating that VEGFR3 expression in human lung adenocarcinoma is primarily found in tumor cells than in stromal and adjacent cells. VEGFR3 expression was also found in blood vessels. Scale bars, 20 µm. (C) Semi-quantitative RT-PCR and qRT-PCR evaluation of VEGF-C mRNA, VEGF-D mRNA and VEGFR3 mRNA are presented. The co-cultured A549 cells (CO-A549) had a higher expression level of VEGF-C mRNA and VEGF-D mRNA than that of the A549 cells alone. (D) Western blot analysis confirmed a high protein expression of VEGF-C and VEGFR3 in CO-A549 cells. *P<0.05, **P<0.01, ***P<0.001. VEGFR, vascular endothelial growth factor receptor; IHC, immunohistochemistry.