Figure 4.

Effect of BSGLEE on cell migration in HCT116 cells. (A) Cell motility upon BSGLEE treatment as determined by wound-healing assay. HCT116 cells were exposed with BSGLEE at 0, 1.6, 4.0 and 10.0 mg/ml for 12 and 24 h, and the pictures were captured by the inverted microscope. (B) BSGLEE inhibited HCT116 cell migration as determined by Transwell experiment. BSGLEE-treated HCT116 cells and untreated HCT116 cells were allowed to migrate in Transwell chambers for 24 h, and the migrated cells were fixed, stained and photographed (magnification, ×100). (C) Histogram of the number of migratory cells, which was counted in five chosen areas. (D-F) Quantification of the mRNA levels of migration-related genes after treatment with BSGLEE as determined by qRT-PCR. Data are presented as mean ± SD of three independent experiments, *P<0.05, **P<0.01.