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. 2018 Jan 23;7:e29913. doi: 10.7554/eLife.29913

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© 2017, Fouad et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (A) Top panels: assessment of ablations using a Pacr-2::mCherry label. After removal of dorsal B motor neurons (Punc-17β::PH::miniSOG), pairs of motor neurons – each corresponding to one VA and one VB type neuron – are visible along the VNC (blue arrows). Neither the DNC (red arrows) nor the DB or DA commissures (red arrowheads in mock) are visible. Scale bars: 100 μm. Bottom panels: Corresponding examples of worm locomotion after removal of DB (via miniSOG) and VB (via IR laser) motor neurons. Removal of DB always resulted in tail paralysis in a coiled position, but a minority of worms were able to generate a rhythmic midbody wave. Additional removal of VB motor neurons by laser ablation completely prevented 2FU and resulted in severe tail paralysis. (B,C) 2FU, as assayed by frequency measurements (B) or blinded, randomized scoring (C), is sharply reduced or eliminated by removal of DB, VB, or both. Head and mid-body frequencies were measured at body coordinates 15 and 60, respectively. Error boxes in (B) are the mean and 95% confidence interval of the mean. Error bars in (C) are standard error of the sample proportion. Numbers above each bar in (C) indicate the number of trials scored 2FU over the total number of trials for the condition; each individual worm contributed between one and five trials (3.6 on average). (***) p<0.001; paired t-test.

Figure 5—figure supplement 1. — (A) Top panels: assessment of ablations using a Pacr-2::mCherry label. After removal of dorsal B motor neurons (Punc-17β::PH::miniSOG), pairs of motor neurons – each corresponding to one VA and one VB type neuron – are visible along the VNC (blue arrows). Neither the DNC (red arrows) nor the DB or DA commissures (red arrowheads in mock) are visible. Scale bars: 100 μm. Bottom panels: Corresponding examples of worm locomotion after removal of DB (via miniSOG) and VB (via IR laser) motor neurons. Removal of DB always resulted in tail paralysis in a coiled position, but a minority of worms were able to generate a rhythmic midbody wave. Additional removal of VB motor neurons by laser ablation completely prevented 2FU and resulted in severe tail paralysis. (B,C) 2FU, as assayed by frequency measurements (B) or blinded, randomized scoring (C), is sharply reduced or eliminated by removal of DB, VB, or both. Head and mid-body frequencies were measured at body coordinates 15 and 60, respectively. Error boxes in (B) are the mean and 95% confidence interval of the mean. Error bars in (C) are standard error of the sample proportion. Numbers above each bar in (C) indicate the number of trials scored 2FU over the total number of trials for the condition; each individual worm contributed between one and five trials (3.6 on average). (***) p<0.001; paired t-test.