Table 1. Constructs and transgenic arrays generated or acquired for this study.
| Purpose | Plasmid | Description |
Host strain/
Injection marker |
Transgene | Strain |
|---|---|---|---|---|---|
| Neuron Ablation | pJH2843 |
Punc-4::tomm20::miniSOG-SL2::RFP (A-MNs/others)* |
lin-15/Lin-15 | hpIs366* | ZM7690* |
| hpIs371* | ZM7691* | ||||
| pJH2842 |
Pacr-5::tomm20-miniSOG-SL2::RFP (B-MN/others) |
lin-15/Lin-15 | hpIs372 | ZM7798 | |
| pJH2844 |
Punc-25::tomm20-miniSOG-SL2::RFP (D-MN/others) |
lin-15/Lin-15 | hpIs376 | ZM7696 | |
| pJH2827 |
Pnmr-1::tomm20-miniSOG-SL2::RFP (AVA/E/D/others) |
lin-15/Lin-15 | hpIs321 | ZM7054 | |
| pJH2890 |
Plgc55B::tomm20::miniSOG-SL2::RFP (AVB/others) |
lin-15/Lin-15 | hpIs331 | ZM7297 | |
| † |
Psra-11::tomm20-miniSOG/
Psra-11::RFP (AVB/others) |
† | juIs440† | CZ19093 † | |
| pJH3626 |
Pacr-2(s)::tomm20-miniSOG-SL2::RFP (A-/B-MN-specific) |
lin-15/Lin-15 | hpIs583 | ZM9062 | |
| pJH3458 |
Pttr-39::tomm20-miniSOG-SL2::BFP (D-MN-specific) |
lin-15/Lin-15 | hpIs590 | ZM9123 | |
| pJH3409/pJH3410 |
Pceh-12::tomm20-miniSOG-SL2::BFP/ Punc-129(DB)::tomm20-miniSOG-SL2::BFP (B-MN-specific) |
lin-15/Lin-15 | hpIs481 | ZM8607 | |
| pJH3449/pJH3453/pJH3456 | Plgc-55B::tomm20-miniSOG-SL2::BFP/ Pnmr-1::tomm20-miniSOG-SL2::BFP/ Pacr-5::tomm20-miniSOG-SL2::BFP (all premotor IN/B MN/others) |
lin-15/Lin-15 | hpIs603 | ZM9176 | |
| Neuron ID | pJH1841 | Pacr-2::RFP | dpy-20/Dpy-20 | qhIs4 | YX146 |
| Optogenetic Stimulation of Neurons | pJH1697 |
Pglr-1::ChR2(H134R)::YFP (AVA/AVE/AVD/others) |
lin-15/Lin-15 | hpIs166 | ZM4624 |
| pJH2690/pJH2673 |
Prig-3::FRT ::ChR2(H134R)::RFP/ Pnmr-1::FLP (AVA-specific) |
lin-15/Lin-15 | hpIs270 | ZM6804 | |
| pJH3558 |
Punc-4::Chrimson::Cherry (A-MNs/others)* |
lin-15/Lin-15 | hpIs569* | ZM8958* | |
| pJH3577 |
Pceh-12::Chrimson::Cherry (VB-MNs) |
lin-15/Lin-15 | hpIs578 | ZM8983 | |
| UNC-2 Localization | pJH3931/pJH3932 | sgRNA and repair template for GFP insertion at the unc-2 locus |
N2 | hp858 | ZM9583 |
| UNC-2 Rescue | pJH3916 | Punc-4::GFP::UNC-2 (WT) |
unc-2(e55); hpIs603; hpIs459/
Pmyo-2::RFP |
hpEx3856 | ZM9495 |
| pJH3917 | Punc-4::GFP::UNC-2 (L653F; gf) |
unc-2(e55); hpIs603; hpIs459/
Pmyo-2::RFP |
hpEx3859 | ZM9498 | |
|
AVA-specific
UNC-7 Rescue |
pJH4119/pJH2673 |
Prig-3::FRT::UNC-7::GFP/ Pnmr-1::FLP (AVA) |
unc-7(e5)/
Pmyo-3::RFP |
hpEx3969 | ZM9816 |
| Calcium Imaging | pJH3137 |
Punc-4::GCaMP6::RFP*
(A-MNs/others) |
lin-15/Lin-15 | hpIs459a | ZM8428* |
| hpIs460* | ZM8429* | ||||
| ‡ |
Pmyo-3::GCaMP3::RFP (body wall muscles) |
‡ | ljIs131‡ | ZM7982‡ |
*In adults, Punc-4 expression is more prominent in VC-MNs than A-MNs. We did not observe rhythmic calcium signals in VCs as in A-MNs (Gao and Zhen, unpublished). Mini-SOG ablation was performed in late L2 larvae; optogenetic stimulation in young L4 larvae, to ensure A-MN-specific ablation and activation.
†Acquired. Information about the construct, transgene and strain was described in Qi, Y. B. et al. Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG. Proc Natl Acad Sci U S A 109, 7499–504 (2012).
‡Acquired. Information about the construct, transgene and strain was described in Butler, V. J. et al. A consistent Muscle activation strategy underlies crawling and swimming in Caenorhabditis elegans. J R Soc Interface 12, 20140963 (2015).