Table 2. Additional strains that were generated using the transgenic arrays in Table 1.
Strain | Genotype | Purpose/Notes | Figure/Video |
---|---|---|---|
For behavior analyses upon sparse A-MN ablation | |||
YX167 | hpIs366/qhIs4; qhIs1* | * A transgene irrelevant to this study | Figure 3; Video 3 |
YX148 | qhIs4; qhIs1* | * A transgene irrelevant to this study | Figure 3; Video 3 |
For behavior and electrophysiology analyses upon neuron ablation | |||
ZM7971 | hpIs321; hpIs331 | All premotor IN ablation | Figure 1; Video 1 |
ZM9133 | hpIs583 hpIs589 | A-/B-/D-MN ablation | Figure 1 |
ZM8415 | hpIs321; juIs440 | All premotor IN ablation | Figure 1—figure supplement 1 |
ZM7862 | hpIs321; hpIs331; hpIs372 | All premotor IN/B-MN ablation | Figure 2; Video 2; Figure 4 |
ZM7870 | hpIs321; hpIs331; hpIs371 | All premotor IN/A-MN ablation | Figure 2; Video 2 |
ZM7921 | hpIs321; hpIs331; hpIs376 | All premotor IN/D-MN ablation | Figure 2; Video 2 |
ZM8668 | unc-2(e55; lf); hpIs321; hpIs331; hpIs372 | All premotor IN/B-MN ablation | Figure 8 |
ZM9022 | unc-2(hp647; gf); hpIs321; hpIs331; hpIs372 | All premotor IN/B-MN ablation | Figure 8 |
ZM9999 | unc-7(e5); hpIs603 | All premotor IN/B-MN ablation | Figure 9—figure supplement 1 |
For Ca22+imaging and/or behavior analyses | |||
ZM7465 | hpIs321; hpIs331; ljIs131 | Muscle Ca2+ imaging, upon all premotor IN ablation |
Video 1 |
ZM9228 | hpIs603; hpIs459 | A-MN Ca2+ imaging, upon all premotor IN/B-MN ablation |
Figures 4 and 5; Video 4 |
ZM9229 | unc-2(e55; lf); hpIs603; hpIs459 | A-MN Ca2+ imaging and behavior, upon all premotor IN/B-MN ablation |
Figure 7 |
ZM9495 | unc-2(e55; lf); hpIs603; hpIs459; hpEx3856 | A-MN Ca2+ imaging, UNC-2 (WT) rescue in A-MN |
Figure 7—figure supplement 2 |
ZM9498 | unc-2(e55; lf); hpIs603; hpIs459; hpEx3859 | A-MN Ca2+ imaging, UNC-2 (GF) rescue in A-MN |
Figure 7—figure supplement 2 |
ZM9231 | unc-2(hp647; gf); hpIs603; hpIs459 | Same as above | Figure 7 |
ZM9557 | unc-13(s69; lf); hpIs603; hpIs459 | Same as above | Figure 7 |
ZM8654 | unc-7(e5; lf); hpIs460 | Same as above | Figure 9 |
ZM9885 | unc-7(e5; lf); hpIs460; hpEx3966 | Same as above | Figure 9 |
ZM9657 | unc-7(e5; lf); hpIs603; hpIs460 | Same as above | Figure 9—figure supplement 1 |
For optogenetic stimulation and electrophysiology analyses | |||
ZM7920 | hpIs321; hpIs270 | AVA ChR2 stimulation, upon AVA/AVE/AVD/others ablation |
Figure 6 |
ZM7861 | hpIs331; hpIs270 | AVA ChR2 stimulation, upon AVB/others ablation |
Figure 6 |
ZM8408 | hpIs371; hpIs270 | AVA ChR2 stimulation, upon A-MN/others ablation |
Figure 6 |
ZM9093 | hpIs481; hpIs166 | AVA/others ChR2 stimulation, upon B-MN/others ablation |
Figure 6 |
ZM7615 | unc-2(e55; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM6646 | unc-2(ra612; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM7608 | unc-36(e251; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM7780 | calf-1(ky867; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM6646 | unc-79(e1068; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM7533 | unc-80(e1069; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM7509 | elg-19(n582; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 7—figure supplement 1 |
ZM6639 | unc-13(e1091; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 9 |
ZM4728 | unc-7(e5; lf); hpIs166 | AVA/others ChR2 stimulation | Figure 9 |