Figure 3.
Sevoflurane inhibits movement behavior and increases the expression level of the GABAA-R ε subunit. The movement behavior of mice was measured using the CatWalk Automatic Gait Analysis system; images were captured of (A) the mice walking and (B) the footprints under the glass. (C) The feet were labeled with different colors for footprint recordings. (D) The footprints were converted to heat maps and (E) the individual footprint heat maps were amplified and analyzed. (F) The cerebellar slices were subjected to an immunohistochemistry assay for GABAA-R ε subunit detection and the amount of GABAA-R ε subunit-positive cells was positively associated with the day of repeated inhalation of sevoflurane; 24 days was the maximal effect time. (G) The expression level of the GABAA-R ε subunit was measured by western blotting. GABAA-R, γ-aminobutyric acidA receptor; Con, control.