Figure 1.
β-ecdysterone reverse Dex-induced suppression of osteoblast differentiation. (A) After 14 days, osteoblast were fixed and stained, and images were captured (magnification, ×100). (B) Osteoblasts were treated with Dex at various concentrations (10−9−10−4 mol/l), and ALP activity was measured. (C) Osteoblasts were treated with Dex (10−6 mol/l) in the presence or absence of β-ecdysterone at various concentrations (10−7−10−5 mol/l), and ALP activity was measured. ***P<0.001, vs. control group; ##P<0.01 and ###P<0.001 vs. Dex group. Dex, dexamethasone; Ale, alendronate; ALP, alkaline phosphatase.