Figure 1.

Production of αIL-39. Purified mouse IL-39 was used for immunization of rabbits and production of αIL-39. (A) Purification of αIL-39 was performed by protein A chromatography. Blue, green and grey lines represent the UV absorption value, column pressure and pH values, respectively. (B) The binding of αIL-39 with recombinant mouse IL-39 (p19/Ebi3) was confirmed by ELISA. Commercial anti-mouse p19 antibody was used as a control. αIL-39, anti-mouse interleukin-39 polyclonal antibodies; OD, optical densisty; Ab, antibody.