Figure 6.

Treatment with αIL-39 reduces infiltrating inflammatory cells and restores the structure of the glomerular region in MRL/lpr mice. (A) Kidney sections were stained with hematoxylin and eosin on day 7 following intravenous injection of 400 µg preimmune IgG (control) or αIL-39 into 6-month-old female MRL/lpr mice (6 mice/group). Red arrows indicate glomeruli and blue arrows indicate infiltrating inflammatory cells. Scale bar, 100 µM (upper panel) and 50 µM (lower panel). (B) Glomerular lesion scoring of kidneys was assessed in a blinded-manner using a four-point scale, and the results are expressed as the mean ± standard error of the mean from 10 glomeruli per kidney from both kidneys of 10 mice per group. ***P<0.001 (two-tailed Student's t-test). αIL-39, anti-mouse interleukin-39 polyclonal antibodies.