Table II.

Primer sequences used in bisulfite-specific polymerase chain reaction and DNA sequencing for CpG methylation in gene promoters.

Gene	Forward primer (5′→3′)	Reverse primer (5′→3′)	Sequencing primer (5′→3′)
AT1aR	TGGGAGGGATTGGATGATGT	Biotin-AAACCCCTAAACTAAAACTCACCAA	GGAGAGGATTAGTGGTTTG
AT1bR	TTTGTTAAGGGAGGGGTTAGGA	Biotin-CCTACTACCTAAAATCCAAACTACCTACA	GGGAGGGGTTAGGAG
ATRAP	TGGTAGAGGTTTAGGTAGTAGTAGGAGT	Biotin-CCAACTCCAAAACAAACTTCCT	GTTTTGTAGTAAGGGTAAGT

AT1aR, angiotensin II type 1 receptor subtype a; ATRAP, angiotensin II type 1 receptor-associated protein.