Skip to main content
. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 29;200(3):1133–1145. doi: 10.4049/jimmunol.1701272

Fig. 3. CD8+ TRM phenotype cells can form and persist at similar numbers in salivary glands from MCMV infected and naïve mice.

Fig. 3

(A) Schematic of the experimental design. Naïve mice, or mice infected 11 days earlier with MCMV (via the i.p. route) were seeded with 3×106 in vitro activated OT-Is. The recipients were sacrificed at 4 and 31 days after transfer. B) Absolute number of OT-Is in the parenchyma of the SG, LG, KDN and from the CD8β+ cells of the SPL. C and D) Frequency of CD103- and CD69-expressing OT-Is in the parenchyma of the SG, LG and KDN at 31 days after transfer. Data are combined from 2 experiments (n= 7 naïve and 6 infected recipients at day; n=6 naïve and 6 infected at day 31). D) Concatenated FACS plots, of CD103 and CD69 expression of the OT-I T cells in the SG from one representative experiment (n=3 naïve and n=3 infected recipients) with mean ± SEM values considering all the experiments. Error bars represent the SEM and the statistical significance in (B) was measured by unpaired t-test after log10 transformation of absolute numbers (***p<0.001).