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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Immunol. 2017 Dec 29;200(3):1133–1145. doi: 10.4049/jimmunol.1701272

Fig. 6. MCMV-specific CD8+ T cells migrate towards multiple chemokines.

Fig. 6

Mice were sacrificed 7 days after MCMV infection (i.p.). CD8+ T cells were enriched and used to performed transwell migration assays with multiple chemokines. Assays were performed with replicates and media control was included in each assay to account for plate-to-plate variation. (A) Absolute numbers of MCMV-specific T cells (M45-Tetramer+ plus M38-Tetramer+) that migrated towards the indicated chemokine in comparison to control wells with media alone. Data are from a single experiment performed in triplicate or quadruplicate, and representative of 2 to 5 experiments per chemokine. Statistical significance was determined using paired t-test (*p<0.05; **p<0.01; ***p<0.001). (B) Fold change of migration in comparison to media. Pooled data from 2 to 4 independent experiments indicating the fold change of migration of MCMV-specific cells (M45-Tetramer+ plus M38-Tetramer+) in response to the indicated chemokines at the concentrations shown in (A).