OVA sensitized WT C57BL/6 mice received challenges with 0.1% OVA on three successive days. The indicated groups of mice received 2.2 nmol of LTC4, N-methyl LTC4, or a vehicle control intranasally 12 h prior to each OVA challenge. A. BAL fluid total cell counts (top), percentage eosinophils (middle) and total eosinophil counts (bottom) from mice euthanized 24 h after the last challenge. B. Numbers of ILC2s in the lungs of the indicated groups. C. Levels of IL-5 (top) and IL-13 (bottom) proteins in lysates of the lungs from the same mice as in B. Results are from 10 mice/group. *** P < 0.001, ** P < 0.01, * P < 0.05.