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. 2018 Jan 19;9:2. doi: 10.3389/fimmu.2018.00002

Figure 2.

Figure 2

Phenolic glycolipids (PGLs) reduce LPS/IFN-γ-induced production of iNOS in a CR3-dependent manner. (A) Differential production of NO by bone marrow-derived macrophages (BMDMs) pretreated with increasing concentrations of PGL-1 or vehicle (Veh) for 24 h prior to a 24 h stimulation with 1 µg/ml LPS and 100 U/ml IFN-γ. Data are mean NO levels ± SEM (n = 4), expressed as fold changes relative to non-treated, stimulated controls. *P < 0.05, Mann–Whitney test comparing PGL-1-treated to vehicle controls at each concentration. (B) As in (A) with 25 µM PGL-1 or 25 µM PGL-tb; unstim: non-treated, non-stimulated controls. (C) As in (A), with increasing concentrations of dimycocerosates (DIMs). (D) Differential induction of iNOS in BMDMs exposed to 25 µM PGL-1 or vehicle (Veh) for 24 h prior to a 24 h stimulation with LPS/IFN-γ. Controls are non-treated, non-stimulated cells (unstim). Data are mean fluorescence intensities (MFI) ± SEM (n = 3). (E) As in (D) in Itgam−/− BMDMs. Data shown are from one experiment repeated twice with similar results. Statistical comparisons in (B,D,E) were performed with repeated measures ANOVA with Tukey post hoc test, relative to the vehicle-treated, LPS/IFN-γ-stimulated group. *P < 0.05, **P < 0.01, ***P < 0.001.