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. 2018 Jan 19;9:2. doi: 10.3389/fimmu.2018.00002

Figure 5.

Figure 5

Phenolic glycolipid (PGL)-1-mediated decrease in TRIF impairs downstream signaling events. Quantitation of mRNAs in bone marrow-derived macrophages (BMDMs) treated with 25 µM PGL-1 or vehicle (Veh) for 24 h prior to 6–24 h of stimulation with LPS/IFN-γ: (A) Nos2 (iNOS); (B) Arg1 (Arginase 1); (C) IL6 (IL-6); (D) CXCL10 (CXCL10); (E) Ifnb1 (IFN-β); (F) Tnf (TNF-α). Data are relative mean transcript levels (n > 3) ± SEM. *P < 0.05, **P < 0.01, Student’s t-test, relative to RPL19 house-keeping gene. They are representative of at least two independent experiments with similar results. Production of IFN-β (G) and TNF-α (H) by BMDMs pretreated with 25 µM PGL-1 for 24 h prior to 6–24 h of stimulation with LPS (n = 6) or LPS/IFN-γ (n = 2). Data in (G,H) are mean cytokine concentrations from biological replicates ± SEM. **P < 0.01, Mann–Whitney test comparing PGL-1-treated cells to vehicle controls.