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. 2018 Jan 19;8:2016. doi: 10.3389/fimmu.2017.02016

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Copyright © 2018 Yang, Wei, Hermosilla, Taubert, He, Wang, Gong, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histone and myeloperoxidase (MPO) in Neospora caninum tachyzoite-triggered caprine monocyte-ETs structures. Caprine monocytes were stimulated with N. caninum tachyzoite (ratio: 1:1) for 90 min. Detection of DNA decorated with of H3 and MPO within the monocyte-ETs structures were examined using a laser scanning confocal microscope. (A) Control histone (green). (G) Histone (green) in monocyte-ETs structures. (D) Control MPO (green). (J) MPO (Green) in monocyte-ETs structures. (B,E) Control DNA (red). (H,K) DNA within these network structures was stained with Sytox Orange (red). (C,F,I,L) Respective merge of DNA decorated with histone and MPO. Three independent experiments were carried out. White arrows showed caprine monocyte-ETs structures triggered by N. caninum.