Figure 3.

Dose dependency of Neospora caninum tachyzoite-triggered extracellular traps (ETs) in caprine monocytes. Caprine monocytes were stimulated with N. caninum tachyzoite (monocytes:tachyzoite = 1:3, 1:6, and 1:12) for 60 min. Zymosan (1 mg/ml) was used as positive controls. Adult healthy caprines (n = 3) were bleed by puncture of the femoral vein and blood was collected. The formation of caprine monocytes ETs was quantified using Sytox Green, a DNA binding dye. Values are presented as mean ± SD (n = 3). P-values of <0.05 were considered significant (*P < 0.05, **P < 0.01, and ***P < 0.001).