Skip to main content
. 2018 Jan 19;9:6. doi: 10.3389/fphys.2018.00006

Figure 4.

Figure 4

Blocking AQP4 and / or NKCC1 reduced spinal cord water content and tissue destruction 48 h after SCI. Spinal cord water content (A) and LDH activity of cerebrospinal fluid (B) were significantly reduced by TGN-20 and bumetanide after SCI, TGN-20, and bumetanide additively reduced spinal cord edema and cerebrospinal LDH activity. (C) HE and methylene blue stainings showed that TGN-20 and bumetanide relieved SCI-induced tissue degeneration (scale bar 50 μm). Quantitative analyses of cavity area (D) and dendrite numbers per field (200x) (E) NC, normal control (uninjured spinal cords); BU, bumetanide; TGN, TGN-020; HE, hematoxylin and eosin staining; MB, methylene blue staining. N = 7. *p < 0.05 compared to NC; #p < 0.05 compared to SCI; ˆp < 0.05 compared to BU; +p < 0.05 compared to TGN.