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. 2018 Jan 19;9:6. doi: 10.3389/fphys.2018.00006

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Copyright © 2018 Yan, Liu, Wang, Li, Chen and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bumetanide and TGN-020 inhibited SCI-induced upregulation of AQP4 and NKCC1. (A) The mRNA levels of AQP4 and NKCC1 of rat spinal cord 48 h after SCI were analyzed by qPCR. (B) The changes of AQP4 and NKCC1 protein levels in rat spinal cord 48 h after SCI with or without TGN-020 and/or bumetanide treatment were assessed by western blot. (C) Quantitative analysis of AQP4 and NKCC1 protein levels in SCI rats after TGN-020 and/or bumetanide treatments. (D) Histoimmunofluorescence detection of changes of AQP4 (green) and NKCC (red) protein levels of injured rat spinal cord tissue treated with TGN-020 and/ or bumetanide (scale bar 50 μm). NC, normal control (uninjured spinal cords); BU, bumetanide; TGN, TGN-020. N = 7. ^*p < 0.05 compared to NC; ^#p < 0.05 compared to SCI; ˆp < 0.05 compared to BU; ⁺p < 0.05 compared to TGN.