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. 2018 Jan 19;8:1998. doi: 10.3389/fimmu.2017.01998

Figure 3.

Figure 3

Effect of mixing mouse IgG induced by vaccination against Pfs25, Pfs230C or Pfs28 in standard membrane-feeding assay (SMFA). Total IgG was purified from pooled day 70 serum from mice vaccinated with chimpanzee adenovirus 63 (ChAd63)–modified Vaccinia Ankara (MVA) expressing Pfs25, Pfs230C, or Pfs28. The purified IgG from mice vaccinated with Pfs25 was mixed with IgG from mice vaccinated with Pfs230 [(A,B) the anti-sera used to purify both anti-Pfs25 and anti-Pfs230 IgGs were from experiment 3]. In addition, IgG from mice vaccinated with Pfs25 was mixed with IgG from mice vaccinated with Pfs28 [(C) both IgGs from experiment 4] at different concentrations. These IgG mixtures or IgG against the single antigens were fed to Anopheles stephensi mosquitoes in SMFA. Data points represent the number of oocysts in individual mosquitoes, and the lines show the arithmetic mean. IgG from mice immunized with ChAd63–MVA expressing green fluorescent protein were used as a negative control (the first left column in each panel). There are significant differences in oocyst density among single IgGs and IgG [p < 0.0001 (A), p < 0.0001 (B), and p < 0.0001 (C) by Kruskal–Wallis tests], and p values (Dunn’s multiple comparison posttests) are shown when there are significant differences between mixture and single IgG in each panel.