Figure 5.

Immunogenicity and transmission reducing ability in standard membrane-feeding assay (SMFA) induced by vaccination with chimpanzee adenovirus 63 (ChAd63)–modified Vaccinia Ankara (MVA) expressing single antigen, dual-antigen, or IMX313 vectors in mice. Day 70 antigen-specific IgG responses in mice after immunization with ChAd63–MVA expressing single antigens Pfs25 or Pfs230C and dual-antigen antigens linked with glycine–proline or 2A (experiment 6, n = 5/group). Another group of mice were immunized with Pfs25-IMX313. The figure shows the total IgG response against Pfs25 (A) and Pfs230C (B). Response was measured by standardized ELISA and is expressed in antibody units. Kruskal–Wallis tests [p = 0.0107 (A) and p = 0.4249 (B)] followed by Dunn’s tests were performed; p value by Dunn’s test is shown. Pfs25-2A-Pfs230C was eliminated from these statistical tests as only three mice remained at the end of the study. Total IgG was purified from the pooled day 70 serum, and each IgG was tested at two to five different total IgG concentrations each in two to three independent SMFA assays (Table S4 in Supplementary Material). The square root of anti-Pfs25 AU (C) in the feeder is shown on the x-axis. The log-transformed mean oocyst ratio between test and control (LMR) are plotted on left side of y-axis, and the associated % inhibition in oocyst density (TRA) is shown on the right side of the y-axis.