Figure 6.

Interleukin-1 receptor type I-deficient (IL-1RI^−/−) mice have fewer monocyte-derived dendritic cells (DCs) and macrophages in the lungs after Cryptococcus neoformans 52D infection. Lung cell suspensions from uninfected and infected mice were stained with fluorochrome-labeled antibodies and analyzed by flow cytometry as described in Section “Materials and Methods.” (A,B) Percentage of DC and macrophage subsets at 0, 7, 14, and 21 days postinfection. (C–E) Total number of mDCs, AMs, and ExMs at 0, 7, 14, and 21 days postinfection. (F) Representative plots of M1 (CD11b⁺, iNOS⁺) and M2 (CD11b⁺, CD206⁺) polarized macrophages in wild-type (WT) and IL-1RI^−/− mice at 14 days postinfection. (G) Percentage of M1- and M2-polarized macrophages in WT and IL-1RI^−/− mice at 14 days postinfection. (H) Mean fluorescence intensity (MFI) of CD206 expression on macrophages in WT compared to IL-1RI^−/− mice and (I) Upregulation of CD206 in AMs and ExMs in IL-1RI^−/− compared to WT mice at 14 days postinfection. (J) MFI and (K) upregulation of CD80- and CD86-positive cells derived from ExMs at 14 days postinfection is shown; (I,K) IL-1RI^−/−, gray filled lines; WT, white filled solid lines; uninfected mice, dashed lines. Data are shown as mean ± SEM and representative of two independent experiments (n = 4 mice/strain/time point). *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.