Fig. 4. Biocontrol genes quantification, using a reverse transcription PCR assay expressed in arbitrary units. A, B, PCR products visualized in 1% agarose gel electrophoresis and stained with ethidium bromide of Bacillus subtilis strain ALICA: lines 1 and 2, subtilosin (360 bp) and subtilisin (672 bp) gene; 3 and 4, protease (290 bp) and β-1,3-glucanase (415 bp). The values are expressed as mean ± SD of the four replicated samples. Asterisk indicates statistically significant differences between treatments (p < 0.05).