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. 2018 Jan 23;8:1420. doi: 10.1038/s41598-018-19300-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Construction and generation of H5CmTERT promoter-regulated oncolytic adenoviruses. (a) A schematic representation of the genomic structures of oncolytic adenoviruses used in this study. Rb7Δ19 contains retinoblastoma (Rb)-binding site-mutated E1A gene and E1B 55 kDa gene, but lacks E1B 19 kDa gene, and E1A expression is controlled by endogenous adenovirus promoter. H5CmTERT-Ad has a backbone similar to that of Rb7Δ19 but replicates under the control of the H5CmTERT promoter rather than the endogenous adenovirus promoter. H5CmTERT-Ad/TRAIL expresses stTRAIL from the adenovirus E3 region of the H5CmTERT-Ad backbone (★ indicates a mutation of the Rb-binding site of E1A). (b) U87MG human glioblastoma cells were infected with Rb7Δ19, H5CmTERT-Ad, or H5CmTERT-Ad/TRAIL at multiplicity of infection (MOI) of 0.5. The concentration of stTRAIL in the culture supernatant was measured by an ELISA. Data are presented as mean ± SD of triplicate experiments. **P < 0.01.