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. 2018 Jan 23;8:1420. doi: 10.1038/s41598-018-19300-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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stTRAIL-mediated potent induction of apoptosis. (a) U87MG or (b) U251N cells were treated with 1 μM of camptothecin (CPT), Rb7Δ19, H5CmTERT-Rd19-Ad, or H5CmTERT-Ad/TRAIL. At 48 h post-treatment, a terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay was conducted. The cells were counted (TUNEL-positive vs total cell count) in five independent fields within microscopic images. The data are representative of three independent experiments conducted in triplicate; *P < 0.05, ***P < 0.001. CPT served as a positive control of induction of apoptosis. A representative field from three independent experiments is shown. Original magnification: ×100 and ×400.