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. 2018 Jan 23;8:1420. doi: 10.1038/s41598-018-19300-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological and immunohistochemical analyses of tumour tissues treated with oncolytic adenoviruses. U87MG tumours established in nude mice were treated with PBS, H5CmTERT-Ad or H5CmTERT-Ad/TRAIL on days 1, 3, and 5, and the tumours were harvested on the 3^rd day after administration of the final treatment. (a) Representative sections were stained with hematoxylin and eosin (H & E). The expression levels of TRAIL and E1A were assessed by immunohistochemical analysis. A TUNEL assay was performed to detect apoptosis. Data are representative of three independent experiments. Original magnification: ×200 and ×400. (b) Semi-quantitative analysis of TRAIL-, TUNEL-, or E1A-stained sections in the MetaMorph image analysis software or ImageJ software. All the results are shown as means ± SD; **P < 0.01, ***P < 0.001.