Fig. 3.

Non-hypoxic menstruation decreases endometrial HIF-1α and delays endometrial repair. a Pimonidazole staining in endometrium at T8 from three mice placed in hyperoxic (75% O2, top panel) and three mice in normoxic (21% O2, bottom panel) conditions at the time of progesterone withdrawal. M myometrium, DM decidualised mass, E endometrium. Inset = negative control. Scale bar = 100 μm. b HIF-1α western blot of uterine protein extracts at T8 from mice in 75% O2 (hyperoxia = non-hypoxic endometrium) versus 21% O2 (normoxia = hypoxic endometrium) with densitometry. *P < 0.05, unpaired t-test. c Endometrial histological breakdown/repair score at T8 in mice incubated in hyperoxic and normoxic conditions. Graphs represent percentage of mice at each histological grade per experimental group. White—decidualisation, light grey—early breakdown, medium grey—full breakdown, dark grey—early repair, black—full repair (Supplementary Fig. 2). d Endometrial histological breakdown/repair score at T24 in mice placed in hyperoxic and normoxic conditions. Graphs represent percentage of mice at each histological grade per experimental group. e Vegf and Cxcr4 concentrations in mouse uterine tissue at T8 and T24 in mice incubated in normoxia and hyperoxia. Box and whisker plots: box represents upper and lower quartiles with horizontal line representing the median, whiskers represent minimum and maximum values