Fig. 3.

Localization and PalmB inhibition of mitoDPP-2 in multiple cell lines. a HEK293T cells treated for 30 min with 1 µM Hoechst 33342, 100 nM MitoTracker Deep Red, and either DMSO or 10 µM PalmB, washed, loaded with 250 nM mitoDPP-2 for 10 min, and then analyzed by confocal fluorescence microscopy. b Enlarged portion of image indicated by dotted white line in (a) showing colocalization of mitoDPP-2 with MitoTracker. c Quantification of the relative fluorescence intensity from mitoDPP-2 and MitoTracker in either control or PalmB-treated cells from (a). d MCF-7 cells treated and analyzed identically to conditions in (a). e Enlarged portion of image indicated by dotted white line in (d). f Quantification of the relative fluorescence intensity from mitoDPP-2 and MitoTracker in either control or PalmB-treated cells from (d). g A549 cells treated and analyzed identically to conditions in (a). h Enlarged portion of image indicated by dotted white line in (g). i Quantification of the relative fluorescence intensity from mitoDPP-2 and MitoTracker in either control or PalmB-treated cells from (g). For all imaging, 5 µm scale bar shown. For all plots, statistical analyses performed with a two-tailed Student′s t-test with unequal variance, *P value < 0.05; **P value < 0.005, n = 3, error bars are ± s.e.m