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. 2018 Jan 19;9:3. doi: 10.3389/fmicb.2018.00003

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Copyright © 2018 La Cono, Ruggeri, Azzaro, Crisafi, Decembrini, Denaro, La Spada, Maimone, Monticelli, Smedile, Giuliano and Yakimov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic affiliation of the bacterial 16S rRNA gene sequences, retrieved from the stably nitrifying enrichments ATA, KRY, and SAL5. The tree was constructed by Neighbor-Joining method and Jukes–Cantor distance matrix using MacVector 11.1.2. Non-parametric bootstrapping was performed upon 1,000 iterations to infer tree topology and the nodes with the percentage of bootstrap re-sampling above 70% are indicated. The 16S phylogenetic tree was rooted with Sunxiuqinia elliptica 16S ribosomal RNA gene (GQ200196). The frequencies of all phylotypes of 16S rRNA, present in clone libraries, are evidenced at the end of each sequence as number of corresponding clones, respectively. The scale bar represents the number of fixed mutations per nucleotide position.