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. 2018 Jan 23;8:1400. doi: 10.1038/s41598-018-19664-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of Toxicity Degree of HDAC inhibitors for SH-SY5Y Cells by Flow Cytometry. SH-SY5Y cells were treated with 1a, 3, 4, 5, 6, 8, 9, 10 or 11 for 48 h as indicated in the section of Material and Methods, and their DNA contents were analyzed using flow cytometry. The percentages of the population in the Sub-G₀/G₁, G₀/G₁, S and G₂/M phase are indicated. 0.1%DMSO was used as a control. The experiment was repeated three times and representative histograms are shown.