FIGURE 3.

The circadian clock drives cycles of RPS6 phosphorylation with a peak during the night. Seedlings of wild-type Arabidopsis were entrained in a light-dark cycle for 11 days, then shifted to continuous light for another four and a half days. Sampling began at ZT30 on the second day in continuous light. Seedlings were grown on medium lacking sucrose (A–C) or containing 1% sucrose (D–F). (A,D) Representative immunoblots for S237-P and S240-P and total RPS6. (B,C,E,F) Immunoblot signals for S237-P and S240-P were quantified and normalized against total RPS6 signals from the same time point, followed by median-centering each data series and averaging of the multiple replicates. Error bars show the standard error of the mean from n = 4 replicates for 0% sucrose and n = 5 for 1% sucrose.