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. 2018 Jan 19;9:1. doi: 10.3389/fimmu.2018.00001

Figure 10.

Figure 10

The regulatory effects of tumor necrosis factor (TNF) and IL-6 affect the expression of gp130 and IL-6R and the phosphorylation of signal transducer and activator of transcription (STAT) 3 and STAT6. (A) qRT-PCR analysis of different signaling molecules in M2 macrophages treated with IL-4 and TNF. Results were normalized to the control group and represent means ± SD of three experiments. Statistical analysis was performing using ANOVA with Tukey’s posttest, and the results were considered significant with a *p < 0.05, **p < 0.01 when compared to the control group, and #p < 0.05 and ##p < 0.01 when compared to the IL-6-treated group. (B) Western Blot analysis of IL-6R, gp130 and b-actin as loading control. Furthermore, the transcription factors signal transducer and activator of transcription (STAT) 3 and STAT6 were analyzed as phosphorylated and non-phosphorylated proteins. One of three experiments is shown. (C,D) Changes of IL-6R and gp130 relative to β-actin are shown. (E,F) The ratio of as phosphorylated and non-phosphorylated STAT3 and 6 is depicted. Results were calibrated to the control group value and represent means ± SD of three experiments. *p < 0.05, **p < 0.01 when compared to the control group, #p < 0.05 and ##p < 0.01 when compared to the IL-6-treated group. One-way ANOVA analysis with Tukey’s postcomparison.