Figure 5.

Phenotypic characterization of monocyte-derived macrophages in the liver of B6.TNF^−/− mice. (A) Gating strategy used in these experiments. (B) The expression of IL-6, CD206, and interferon-γ were investigated in the combined population from B6.WT and B6.TNF^−/− mice at day 42 p.i. using flow cytometry. (C) Gene expression of IL-6, CD206, inducible nitric oxide synthase, and arginase-1 relative to β-actin expression in the combined population of B6.WT and B6.TNF^−/− mice at d42 p.i. Each error bar represents the means ± SD from five mice in one experiment, and results were confirmed by further two independent experiments. The p-values were calculated using a two tailed Mann–Whitney U-test (*p < 0.05, **p < 0.01).