Table 1.
Host | Target | Fluorochrome | Clone | Supplier | |
---|---|---|---|---|---|
Primary antibodies | Rabbit | L. major | – | V121 | M. Mack, Regensburg |
Rat | CD11b | Biotin | M1/70 | BD Biosciences | |
Mouse | iNOS | – | 6/iNOS/NOS Type II | BD Biosciences | |
Rat | CD206 | – | MR5D3 | BD Biosciences | |
Rat | IL-6 | Biotin | MP5-32C11 | BioLegend | |
Secondary reagents | Rat | Streptavidin | Alexa Fluor 546 | – | Thermo Fisher Scientific |
Donkey | Rat IgG | Alexa Fluor 647 | – | Thermo Fisher Scientific | |
Goat | Rabbit IgG | Alexa Fluor 488 | – | Thermo Fisher Scientific | |
Goat | Mouse IgG | Alexa Fluor 647 | – | Thermo Fisher Scientific |