Table 1.
Primary and secondary antibodies used in confocal microscopy.
| Host | Target | Fluorochrome | Clone | Supplier | |
|---|---|---|---|---|---|
| Primary antibodies | Rabbit | L. major | – | V121 | M. Mack, Regensburg |
| Rat | CD11b | Biotin | M1/70 | BD Biosciences | |
| Mouse | iNOS | – | 6/iNOS/NOS Type II | BD Biosciences | |
| Rat | CD206 | – | MR5D3 | BD Biosciences | |
| Rat | IL-6 | Biotin | MP5-32C11 | BioLegend | |
| Secondary reagents | Rat | Streptavidin | Alexa Fluor 546 | – | Thermo Fisher Scientific |
| Donkey | Rat IgG | Alexa Fluor 647 | – | Thermo Fisher Scientific | |
| Goat | Rabbit IgG | Alexa Fluor 488 | – | Thermo Fisher Scientific | |
| Goat | Mouse IgG | Alexa Fluor 647 | – | Thermo Fisher Scientific | |