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. 2018 Jan 23;8:1401. doi: 10.1038/s41598-018-19831-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nucleosome affinity of certain region on AOX1 promoter is related to mutant promoter strength. Predicted nucleosome affinity profiles of region (−820 to −540) and (−620 to −430), which was generated by NuPoP software for several variants, were shown in (a) and (d) respectively; the cumulative sum of predicted nucleosome affinity across the region (−820 to 540) was positively related to promoter strength on a proportional basis (b); the cumulative sum of predicted nucleosome affinity across the region (−620 to −430) was negatively related to promoter strength on a proportional basis (d).