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. 2018 Jan 19;9:10. doi: 10.3389/fpls.2018.00010

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Copyright © 2018 Waters, Amundsen and Graef.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Leaf chlorophyll and iron (Fe) concentration in IDC tolerant and sensitive soybean plants. (A) chlorophyll (in SPAD units) and (B) Fe concentration in the first trifoliate leaf. Plants were pretreated with 1 or 25 μM Fe(III)-EDDHA for 6 d then treated with 1 or 25 μM Fe(III)-EDDHA and 0, 5, or 15 mM sodium bicarbonate for 4 d. At low Fe supply, Bic treatment resulted in statistically significantly lower leaf chlorophyll (p < 0.05), and the genotypes were significantly different at 15 mM Bic. At both Fe levels, addition of Bic resulted in statistically significantly lower leaf Fe concentration (p < 0.05), but there were no differences between genotypes.