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. 2018 Jan 23;8:1449. doi: 10.1038/s41598-018-19626-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence comparison between the ASFV p30 gene-specific guide RNA gene sequence of WSL-gRp30 cells (A), the corresponding viral sequences of ASFV-BA71 and ASFV-Kenya1033 (B), and of two escape mutants of ASFV-Ba71VΔTKdsRed isolated after passage on WSL-gRp30 cells (C). A chromatogram indicating nucleotide peaks and quality is shown above the excerpt of the determined cellular sequence, and the deduced p30 amino acid sequences with position numbers are given below the viral gene fragments. Differences to ASFV-BA71 are coloured, and the targeted 20 nt (vertical lines) as well as the following PAM (red rectangle) are indicated.