Table 1.

Oligonucleotide primers used in this study.

Name	Sequence	Position
C9ΔNLS1-MF	GACGATGACGATAAGATG/GACAAGAAGTACAGCATC	1305–1322/1371–1388 [1]
C9ΔNLS2-MF	CTCAGCTGGGAGGCGAC/TAAGAATTCCTAGAGCTC	5455–5471/5520–5537 [1]
gRp30-F	caccGCAAGGGTATACTGAACATC	125,144–125,163 (R) [2]
gRp30-R	aaacGATGTTCAGTATACCCTTGC	125,144–125,163 (R) [2]
X330gRR-F	ATGCTTACCGTAACTTGAAAG	181–201 [1]
X330gRR-R	ATTTGTCTGCAGAATTGGCG	405–424 (R) [1]
CP204L-PSF	CACAAGTTGTGTTTCATGC	125290–125308 (R) [2]
CP204L-PSR	TGAAGATCCACGGTTACCC	124670–124688 [2]

Primers C9ΔNLS1-MR and C9ΔNLSR-MR were reverse complementary to the respective forward primers, and slashes (/) indicate the positions of the deleted NLS encoding sequences. Nucleotides printed in lower case have been added for convenient cloning of the hybridised oligonucleotides. Nucleotide positions refer to the forward or reverse (R) strand of the sequence of [1] pX330-U6-Chimeric_BB-CBh-hSpCas9²⁰, or of [2] ASFV Georgia³⁴.