Table 2.
Characteristics of sequence at mutation sites or rejoined sites of insertion and deletion mutations.
| Type of Mutation | Irradiation material | Homopolymeric sequence | Polynucleotide repeat or microhomology | ||
|---|---|---|---|---|---|
| Total | Breakdown by length | ||||
| −1 | Dry seed | 45% (22/49) | 18% (9/49) | ||
| Seedling | 50% (8/16) | 6% (1/16) | |||
| Del_≥2 bp | Dry seed | 9% (12/129) | 49% (63/129) | 2–9 bp | 48% (38/80) |
| 10–49 bp | 58% (23/40) | ||||
| ≥50 bp | 22% (2/9) | ||||
| Seedling | 5% (2/44) | 43% (19/44) | 2–9 bp | 42% (8/19) | |
| 10–49 bp | 53% (10/19) | ||||
| ≥50 bp | 17% (1/6) | ||||
| +1 | Combined* | 44% (8/18) | 27% (5/18) | ||
| Ins_≥2 bp | Combined* | 29% (5/17) | 76% (13/17) | ||
Numbers in parentheses represent number of mutation events associated with homopolymeric sequences (≥3 bp, e.g. AAAA), polynucleotide repeats (e.g. TATA) or microhomology at mutation site or rejoined site/ total number of mutation events corresponding to each category. Details of sequences are shown in Table S5.
*Combined data from dry-seed irradiation and seedling irradiation.