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. 2018 Jan 23;8:1462. doi: 10.1038/s41598-018-19780-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RT-PCR analysis of P.berghei Pbuis4, Pbuis10, Pbuis24 and Pbcsp transcripts. Templates were derived from gDNA (lane 2), dissected midguts at 11 d pbm sporozoites (lane 3, MG SPZ) and from dissected salivary glands at 21 d pbm (lane 4 SG SPZ). Lane 1 is the negative control without template (NC). The samples were amplified for 35 cycles. Molecular weight of the amplified fragments is indicated to the right. Unprocessed images of the agarose gels are shown in Supplementary Fig. S4b.