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. 2018 Jan 23;8:1404. doi: 10.1038/s41598-018-19783-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evolution of HIV-1 quasispecies within one couple at different follow-up times from the phylogenetic tree. (A–D) The evolution of FP/MP and FW/MW. FP/MP mean the plasma samples of F and M, respectively. FW/MW mean the whole-blood samples of F and M, which were collected from the female (F) and the male (M) partners at various time points. The calculation time required was reduced by including only quasispecies identified more than 50 times.