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. 2018 Jan 23;8:1463. doi: 10.1038/s41598-018-19873-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of LA on cellular copper toxicity. Viability of Huh7 cells determined by WST-1 assay after 24 h of exposure to CuCl₂ (a). Viability of Huh7 cells after 24 h incubation with different concentrations of LA and additional treatment with 50 µM CuCl₂ for next 24 h, measured with WST-1 test. Data are shown as mean ± SD.