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. 2018 Jan 23;8:1402. doi: 10.1038/s41598-018-19915-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEM images of the various growth experiments shown at different magnifications to give a broad perspective of nuclei density and coverage of the SCD surface. Growth experiments are depicted as follows: (a) 1 minute (b) 3 minutes, dark regions are SCD areas without NCD growth. (c) 15 minutes showing uniform coverage (d) 3 hours (e) 3 hour side view showing growth only in the region defined by tungsten mask (f) 6 hours (g) 6 hour side view showing a satellite spherical NCD growth in addition to central second stage defined by tungsten mask.