Table 1.
Oligonucleotide primer sequences and corresponding parameters for qPCR experiments.
| Gene | Reference ID | Nucleotide sequence | Amplicon size | Annealing temperature |
|---|---|---|---|---|
| ACTA2 | NM_001613.2 | 5′-ACA ATG AGC TTC GTG TTG CC-3′ 5′-GAG TCA TTT TCT CCC GGT TGG-3′ |
90 bp | 59 °C |
| CCL2 | NM_002982.3 | 5′-CAG CCA GAT GCA ATC AAT GCC-3′ 5′-TGG AAT CCT GAA CCC ACT TCT-3′ |
190 bp | 58 °C |
| CCL7 | NM_006273.3 | 5′-TGG AGA GCT ACA GAA GGA CCA-3′ 5′-GTG GGG TCA GCA CAG ATC TC-3′ |
94 bp | 58 °C |
| CNN1 | NM_001299.5 | 5′-CGA AGA CGA AAG GAA ACA AGG T-3′ 5′-GCT TGG GGT CGT AGA GGT G-3′ |
186 bp | 62 °C |
| COL1A1 | NM_000088.3 | 5′-AAG AGG AAG GCC AAG TCG AG-3′ 5′-CAC ACG TCT CGG TCA TGG TA-3′ |
91 bp | 60 °C |
| COL1A2 | NM_000089.3 | 5′-CCG GAG ATA GAG GAC CAC GT-3′ 5′-CAG CAA AGT TCC CAC CGA GA-3′ |
132 bp | 60 °C |
| COL3A1 | NM_000090.3 | 5′-GGT GGT TTT CAG TTT AGC TAC GG-3′ 5′-TGA TGT TCT GGG AAG CTC GG-3′ |
106 bp | 59 °C |
| COL4A2 | NM_001846.3 | 5′-AAG GAA TCA TGG GCT TTC CT-3′ 5′-CTC TGG CAC CTT TTG CTA GG-3′ |
204 bp | 60 °C |
| COL6A2 | NM_001849.3 | 5′-GTC ATG AAA CAC GAA GCC TAC G-3′ 5′-CAC CCT TCT GTC CAC GGT AG-3′ |
97 bp | 59 °C |
| CXCL12 | NM_000609.6 | 5′-TCA GCC TGA GCT ACA GAT GC-3′ 5′-CTT TAG CTT CGG GTC AAT GC-3′ |
161 bp | 60 °C |
| CXCL5 | NM_002994.4 | 5′-CAG CGC TCT CTT GAC CAC TA-3′ 5′-GAA CTC CTT GCG TGG TCT GT-3′ |
194 bp | 60 °C |
| GDNF | NM_000514.3 | 5′-GCA GAC CCA TCG CCT TTG AT-3′ 5′-ATC CAC ACC TTT TAG CGG AAT G-3′ |
93 bp | 60 °C |
| HPRT | NM_000194.2 | 5′-CCT GGC GTC GTG ATT AGT GA-3′ 5′-GGC CTC CCA TCT CCT TCA TC-3′ |
163 bp | 60 °C |
| IGFBP3 | NM_001013398.1 | 5′-ACA GCC AGC GCT ACA AAG TT-3′ 5′-CTA CGG CAG GGA CCA TAT TC-3' |
100 bp | 59 °C |
| IL1B | NM_000576.2 | 5′-CTT GGT GAT GTC TGG TCC ATA TG-3′ 5′-GGC CAC AGG TAT TTT GTC ATT AC-3′ |
127 bp | 60 °C |
| IL33 | NM_001199641.1 | 5′-AGG TGA CGG TGT TGA TGG TAA-3′ 5′-AAG GAC AAA GAA GGC CTG GT-3′ |
142 bp | 60 °C |
| IL6 | NM_000600.4 | 5′-AAC CTG AAC CTT CCA AAG ATG G-3′ 5′-TCT GGC TTG TTC CTC ACT ACT-3′ |
159 bp | 60 °C |
| LOX | NM_001317073.1 | 5′-CAC ACA CAC AGG GAT TGA GTC-3′ 5′-AGT CAG ATT CAG GAA CCA GGT-3′ |
147 bp | 60 °C |
| P2RX4 | NM_001256796.1 | 5′-AGA TGC GAC CAC TGT GTG TA-3′ 5′-GTT GAG ACT CCG TTG CTG TG-3′ |
78 bp | 60 °C |
| P2RX7 | NM_002562.5 | 5′-TGT CCC ATT TTC CGA CTA GG-3′ 5′-CCA ACG GTC TAG GTT GCA GT-3′ |
120 bp | 60 °C |
| RPL19 | NM_000981.3 | 5′-AGG CAC ATG GGC ATA GGT AA-3′ 5′-CCA TGA GAA TCC GCT TGT TT-3′ |
199 bp | 60 °C |
| SPP1 | NM_001040058.1 | 5′-TTT TCA CTC CAG TTG TCC CC-3′ 5′-TAC TGG ATG TCA GGT CTG CG-3′ |
109 bp | 59 °C |
| THBS1 | NM_003246.3 | 5′-AGT CGT CTC TGC AAC AAC CC-3′ 5′-AGC TAG TAC ACT TCA CGC CG-3′ |
148 bp | 60 °C |
| TPSAB1 | NM_003294.3 | 5′-GCG ATG TGG ACA ATG ATG AG-3′ 5′-CAA GGT GGT ATT TTG CGT CA-3′ |
102 bp | 60 °C |