Table 1.
Basic characteristic of reads in four libraries and data of sequencing reads mapping to the reference genome.
| Sample name | BSM | GSM | NSM | WSM |
|---|---|---|---|---|
| Raw reads | 99,758,320 | 109,996,464 | 119,299,572 | 136,748,678 |
| Clean reads | 93,987,492 | 103,305,608 | 112,944,804 | 130,967,948 |
| Clean bases | 14.1 G | 15.5 G | 16.94 G | 19.65 G |
| Error rate(%) | 0.02 | 0.01 | 0.02 | 0.01 |
| Q20(%) | 96.91 | 97.1 | 96.96 | 97.6 |
| Q30(%) | 92.47 | 92.81 | 92.57 | 93.88 |
| GC content(%) | 44.08 | 46.57 | 44.08 | 45.16 |
| Total mapped | 70571864 (75.09%) | 81660962 (79.05%) | 84586510 (74.89%) | 100807144 (76.97%) |
| Multiple mapped | 7312694 (7.78%) | 9367467 (9.07%) | 7700828 (6.82%) | 10709954 (8.18%) |
| Uniquely mapped | 63259170 (67.31%) | 72293495 (69.98%) | 76885682 (68.07%) | 90097190 (68.79%) |
| Read-1 | 32615939 (34.7%) | 37203463 (36.01%) | 39517852 (34.99%) | 45957811 (35.09%) |
| Read-2 | 30643231 (32.6%) | 35090032 (33.97%) | 37367830 (33.09%) | 44139379 (33.7%) |
| Reads map to ‘+’ | 31768580 (33.8%) | 36272547 (35.11%) | 38639356 (34.21%) | 45154870 (34.48%) |
| Reads map to ‘−’ | 31490590 (33.51%) | 36020948 (34.87%) | 38246326 (33.86%) | 44942320 (34.32%) |
| Non-splice reads | 41421851 (44.07%) | 50059318 (48.46%) | 52748486 (46.7%) | 58198638 (44.44%) |
| Splice reads | 21837319 (23.23%) | 22234177 (21.52%) | 24137196 (21.37%) | 31898552 (24.36%) |