Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 23;8:1410. doi: 10.1038/s41598-018-19471-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CNF1 increases phosphorylation of HACE1 on Ser-385. (a) Fragmentation spectra of the DS(p)TEITSILLK(+2) peptide showing that Ser-385 is phosphorylated. (b) Protein lysates from HUVECs transfected with HA-HACE1(WT) and treated with CNF1 at 10⁻⁹ M for 24 hours were treated or not with λ-phosphatase (λ-PPase) and analyzed by immunoblot (IB) using the indicated antibodies. IB: actin is used as a loading control. (c) Graph showing levels of pSer-385HACE1 relative to HACE1 total protein levels quantified by densitometry from the IB analysis. Data correspond to the mean ± SD of >3 biological replicates. p value was determined by one-sample t-test. (d) Protein lysates from HUVECs transfected with HA-HACE1(WT) or HA-HACE1(S385A), treated with CNF1 at 10⁻⁹ M for 24 hours and analyzed by IB. (e) Protein lysates from HUVECs transfected with HA-HACE1(WT), treated with CNF1 at 10⁻⁹ M for the indicated times, and analyzed by IB. (f) Protein lysates from HUVECs treated with CNF1 at 10⁻⁹ M for 24 hours. IBs in (b,d,e,f) are cropped from the full-length blots shown in Supplemental Figure S9.