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. 2018 Jan 23;8:1395. doi: 10.1038/s41598-018-19836-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of antibiotic treatment on peripheral PER2::LUC rhythms. Vehicle (water) or mixed antibiotics was administered to mice in drinking water over a month. (A) Average waveform of in vivo peripheral PER2::LUC imaging in the kidney, liver, and submandibular gland (sub gla). (B,C) Peak phase and amplitude of PER2::LUC rhythms. All the values are expressed as mean ± SEM. The number of mice used in this study is indicated in Table S1. More details on the statistics used is presented in Table S2. *p < 0.05, **p < 0.01, vs. vehicle gavaged group (t-test). P value of the two-way ANOVA is indicated in the lower right side of each graph if significant.