Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 23;8:1405. doi: 10.1038/s41598-018-19538-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Aggregated mHtt is conjugated with TAMRA-Ub. (a) Insoluble fraction of Neuro-2A cell lysate 48 hours after transient transfection with Htt-exon1-97Q-H4. After 24 hours expression cells were electroporated with TAMRA-Ub and incubated for additional 24 hours. Formic acid-dissolved inclusion bodies were loaded on a SDS-PAGE and TAMRA signal of ubiquitinated mHtt (asterisks) was detected by fluorescence scan. mHtt proteins were detected on Western blot by anti-HA immunostaining. Non-electroporated cells expressing Htt-exon1-97Q-H4 were used as a control. (b) Confocal images of Neuro-2A cells expressing the mutant 3xR Htt-exon1-97Q-H4. Cells were electroporated with TAMRA-Ub wild type 24 hours after transfection and incubated for additional 24 hours. After fixation cells were stained with an anti-HA antibody and the nuclei were stained with DAPI. Scale bar: 2 µm. (c) Insoluble fractionation of Neuro-2A cell lysate 48 hours after transient transfection with Htt-exon1-97Q-H4 or 3XR Htt-exon1-97Q-H4. Formic acid-dissolved inclusion bodies were loaded on a SDS-PAGE. Htt proteins and ubiquitinated material were detected on Western blot by anti-HA and anti-Ub immunostaining.